Semi Prep HPLC Column, Bidentate C8 300, 5um, 300A. 10mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑SP250‑3M Cogent Bidentate C8™ 300 Semi‑Preparative HPLC Column is a long‑format, wide‑pore semi‑prep column designed for high‑capacity purification and maximum‑resolution separation of macromolecules, including proteins, peptides, and polysaccharides. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, fast equilibration, and very robust column lifetime under demanding preparative workflows.
The stationary phase uses an aliphatic C8 hydrocarbon chain bonded via direct silicon–carbon bonds to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than conventional wide‑pore C8 columns, yet more appropriately balanced for biomolecules than C18, allowing strong yet controlled retention without excessive adsorption. The 300 Å pore structure enables efficient analyte access, improving mass transfer, peak shape, and purification performance for large molecules.
Unlike conventional wide‑pore silica phases, Bidentate C8™ 300 can retain compounds that often show poor or inconsistent retention on traditional materials. It can also retain select hydrophilic macromolecules, particularly when used under HILIC‑like conditions, providing expanded flexibility in preparative method development. The column is also stable at very low pH (below pH 2), supporting aggressive purification methods not possible with standard silica phases.
With a 10 mm internal diameter and 250 mm length, this column provides the highest plate count and loading capacity in the semi‑prep C8™ 300 lineup, making it ideal for maximum‑resolution purification and tight fraction collection windows when processing large sample volumes.
System Note: A specialized semi‑preparative HPLC system (high‑flow pump capacity, appropriate injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size and length.
Why Choose the 10 × 250 mm Bidentate C8™ 300 Format
This configuration is typically selected when:
Large biomolecules require 300 Å pore access
Maximum purification resolution is required
Shorter semi‑prep columns do not fully resolve closely related species
Large sample loads demand high capacity and efficient separation
Wide‑pore C18 causes excessive adsorption or poor recovery
The combination of long column length, wide‑pore structure, and TYPE‑C™ silica hydride chemistry delivers exceptional separation performance and robustness for biomolecule purification.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Direct Si–C bonding for superior durability
Reduced silanol activity and improved peak shape
Faster equilibration than conventional wide‑pore silica columns
Bidentate C8™ 300 Bonding
Enhanced hydrophobic interaction vs standard C8
Balanced retention for proteins and macromolecules
Avoids excessive adsorption seen with C18 phases
Wide‑Pore (300 Å) Architecture
Optimized for proteins, peptides, and polysaccharides
Improved mass transfer and chromatographic efficiency
Supports large‑molecule purification
Maximum‑Resolution Semi‑Prep Geometry
10 mm ID × 250 mm length
Highest plate count and separation power
Supports high mass loading and tight fraction collection
Broad Method Compatibility
Stable at very low pH (below pH 2)
Compatible with aqueous‑dominant mobile phases
Supports HILIC‑like operation for select macromolecules
Typical Applications
Semi‑preparative purification of proteins and peptides
Polysaccharide isolation and purification
Biomolecule fraction collection
High‑resolution wide‑pore reversed‑phase separations
LC‑MS or UV‑based preparative workflows
Scale‑up from analytical or shorter semi‑prep methods
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable